Biotechnology: Principles and Processes NEET PYQs (1994–2026)

Solved Biotechnology: Principles and Processes PYQs

3 recent questions with answers. The full set of 133 is free to practise.

1. NEET 2026HardPrinciples and tools of biotechnology
   Q1

   Match List I with List II:
   
   List-I:
   
   A. Genetically modified organism,
   
   B. Thermostable DNA polymerase,
   
   C. Ti plasmid,
   
   D. pBR322
   
   List-II:
   
   (I) Agrobacterium tumefaciens,
   
   (II) Bt cotton,
   
   (III) Thermus aquaticus,
   
   (IV) Escherichia coli
   
   Choose the correct answer from the options given below:

   • AA-II, B-III, C-I, D-IV
   • BA-II, B-I, C-IV, D-III
   • CA-I, B-IV, C-III, D-II
   • DA-I, B-II, C-IV, D-III

   Show answer & solution

   Correct answer: (A)

   GMO -- Bt cotton (II); Taq polymerase -- Thermus aquaticus (III); Ti plasmid -- Agrobacterium (I); pBR322 -- E. coli (IV). So A-II, B-III, C-I, D-IV.
2. NEET 2026MediumPCR and gel electrophoresis
   Q2

   Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction:

   • AExtension → Annealing → Denaturation
   • BAnnealing → Denaturation → Extension
   • CDenaturation → Extension → Annealing
   • DDenaturation → Annealing → Extension

   Show answer & solution

   Correct answer: (D)

   PCR steps: Denaturation (95°C), Annealing (55°C), Extension (72°C).
3. NEET 2026HardRestriction enzymes and cloning vectors
   Q3

   Which of the following statements are not true regarding
   
   restriction endonucleases?
   
   A. They are called molecular scissors.
   
   B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
   
   C. They cut the DNA only at the centre of the palindromic sites.
   
   D. They remove nucleotides only from the ends of DNA fragments.
   
   E. They recognise specific palindromic base-pair sequences.
   
   Choose the answer from the options given below:

   • AA and B only
   • BA and E only
   • CD and E only
   • DC and D only

   Show answer & solution

   Correct answer: (D)

   C is false (they cut at specific sites, not always centre). D is false (that's exonuclease). So C and D are not true.

Topic-wise question count

Where NEET has asked from Biotechnology: Principles and Processes, most frequent first.

• Restriction enzymes and cloning vectors61
• PCR and gel electrophoresis30
• Principles and tools of biotechnology17
• Recombinant DNA technology steps16
• Bioprocess engineering and downstream processing6
• Transgenic organisms (brief)3

Year-wise Biotechnology: Principles and Processes questions in NEET

Number of questions from this chapter in each year's paper(s).